Trident: A dual oxygenation and fluorescence imaging platform for real-time and quantitative surgical guidance

Despite recent technological progress in surgical guidance, current intraoperative assessment of tissue that should be removed (e.g., cancer) or avoided nerves) is still performed subjectively. Optical imaging a non-contact, non-invasive modality has the potential to provide feedback regarding condition living tissues by either an exogenously administered contrast agent endogenous constituents such as hemoglobin, water, and lipids. As such, optical attractive physiologically structurally relevant information for decision-making real-time during surgery. The Trident platform been designed guidance using state-of-the-art imaging. This capable dual exogenous owing unique filter source combination, allowing take advantage both modalities. makes use quantitative method working spatial frequency domain, called Single Snapshot Properties (SSOP). comply with all standards clinical use. In this manuscript, we first introduce rationale developing platform. We then describe fluorescence modalities where present details design, assess performance on bench. Finally, perform validation vivo preclinical experiment. Altogether, work lays foundation translating technology clinic.